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兔子可溶性E选择素(sE-selectin)ELISA分析试剂盒

  • 更新时间:  2018-01-07
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  • 简单描述
  • 兔子可溶性E选择素(sE-selectin)ELISA分析试剂盒,英文缩写:sE-selectin ,规格:96T/48T,英文名称:sE-selectin ELISA Kit
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兔子可溶性E选择素(sE-selectin)ELISA分析试剂盒,英文缩写:sE-selectin ,规格:96T/48T,英文名称:sE-selectin ELISA Kit
保存条件:低温避光
产品规格:48T/96T
公司自主研发、批量生产的具有高效省时,质优价廉等特点的一款兔子可溶性E选择素(sE-selectin)ELISA分析试剂盒 
1)高效省时,其中IL-2,IL-6, IL-8试剂盒“zui短操作时间”仅为2小时
2)已有多种细胞因子检测试剂盒保质期可达到12个月
3)不同于“进口分装”,出厂经全套质控程序(标准曲线线性质控)
4)37度稳定性试验、批次间、批次内重复性质控等
5)大量现货库存,到货及时
6)采用全进口原料,沿用国际生产质控标准进行批量生产
33600人干扰素诱导T细胞趋化因子(ITAC/CXCL11)ELISA试剂盒Human Interferon inducible T-cell Chemoattractant,I-TAC ELISA Kit96T/48T
33601人CD14分子(CDl4)ELISA试剂盒Human cluster Of differentiation,CDl4 ELISA Kit96T/48T
33602人白细胞共同抗原(LCA/CD45)ELISA试剂盒Human leukocyte common antigen,LCA/CD45 ELISA Kit96T/48T
33603人CD4分子(CD4)ELISA试剂盒Human cluster Of differentiation,CD4 ELISA Kit96T/48T
33604人CXC趋化因子配体16(CXCL16)ELISA试剂盒Human CXC-chemokine ligand 16,CXCL16 ELISA Kit96T/48T
33605人CXC趋化因子受体3(CXCR3)ELISA试剂盒Human CXC-chemokine receptor 3,CXCR3 ELISA Kit96T/48T
33606人γ干扰素诱导蛋白16/p16(IFI16/p16)ELISA试剂盒Human interferon-inducible protein 16,IFI16/p16 ELISA Kit96T/48T
33607人基质细胞衍生因子1a(SDF-1a/CXCL12)ELISA试剂盒Human Stromal cell derived factor 1a,SDF-1a ELISA Kit96T/48T
33608人可溶性CD86(B7-2/sCD86)ELISA试剂盒Human soluble Cluster of differentiation 86,sCD86 ELISA Kit96T/48T
33609人巨噬细胞移动抑制因子(MIF)ELISA试剂盒Human Macrophage Migration Inhibitory Factor,MIF ELISA Kit96T/48T
33610人组织因子途径抑制物(TFPI)ELISA试剂盒Human Tissue factor pathway inhibitor,TFPI ELISA Kit96T/48T
33611人干扰素诱导蛋白10(IP-10/CXCL10)ELISA试剂盒Human interferon-inducible protein 10,IP-10 ELISA Kit96T/48T
33612人巨噬细胞炎性蛋白5(MIP-5)ELISA试剂盒Human macrophage inflammatory protein 5,MIP-5 ELISA Kit 96T/48T
33613人结缔组织生长因子(CTGF)ELISA试剂盒Human connective tissue growth factor,CTGF ELISA Kit96T/48T
33614人B细胞活化因子受体(BAFF-R)ELISA试剂盒 Human B cell activation factorr from the tumor necrosis factor family receptor,BAFF-R ELISA Kit96T/48T
33615人血管内皮细胞生长因子受体3(VEGFR-3/Flt-4)ELISA试剂盒Human Vascuoar endothelial cell growth factor receptor 2,VEGFR-2/Flk-1 ELISA Kit 96T/48T
33616人血管内皮细胞生长因子受体1(VEGFR-1/Flt1)ELISA试剂盒Human Vascuoar endothelial cell growth factor receptor 1,VEGFR-1/Flt1 ELISA Kit 96T/48T
33617人血管内皮细胞生长因子D(VEGF-D)ELISA试剂盒Human Vascular Endothelial cell Growth Factor D,VEGF-D ELISA Kit 96T/48T
33618人血管内皮细胞生长因子C(VEGF-C)ELISA试剂盒Human Vascular Endothelial cell Growth Factor C,VEGF-C ELISA Kit 96T/48T
33619人可溶性肿瘤坏死因子相关凋亡诱导配体(sTRAIL)ELISA试剂盒 Human soluble tumor necrosis factor-related apoptosis inducing ligand,sTRAIL ELISA Kit 96T/48T
33620人肿瘤坏死因子相关凋亡诱导配体4(TRAIL-R4)ELISA试剂盒Human tumor necrosis factor-related apoptosis-inducing ligand 4,TRAIL-R4 ELISA Kit96T/48T
33621人肿瘤坏死因子相关凋亡诱导配体3(TRAIL-R3)ELISA试剂盒Human tumor necrosis factor-related apoptosis-inducing ligand 3,TRAIL-R3 ELISA Kit96T/48T
33622人肿瘤坏死因子相关凋亡诱导配体1(TRAIL-R1)ELISA试剂盒Human tumor necrosis factor-related apoptosis-inducing ligand 1,TRAIL-R1 ELISA Kit96T/48T
33623人肿瘤坏死因子可溶性受体Ⅱ(TNFsR-Ⅱ)ELISA试剂盒Human Tumor necrosis factor soluble receptor Ⅱ,TNFsR-Ⅱ ELISA Kit 96T/48T
33624人肿瘤坏死因子可溶性受体Ⅰ(TNFsR-Ⅰ)ELISA试剂盒Human Tumor necrosis factor soluble receptor Ⅰ,TNFsR-ⅠELISA Kit 96T/48T
33625人基质细胞衍生因子1β(SDF-1β/CXCL12)ELISA试剂盒 Human Stromal cell derived factor 1β,SDF-1β ELISA Kit96T/48T
33626人干细胞因子受体(SCFR)ELISA试剂盒Human Stem Cell Factor Receptor,SCFR ELISA Kit 96T/48T
33627人正常T细胞表达和分泌因子(RANTES/CCL5)ELISA试剂盒 Human regulated on activation in normal T-cell expressed and secreted,RANTES ELISA Kit 96T/48T
33628人神经营养因子4(NT-4)ELISA试剂盒Human Neurotrophin 4,NT-4 ELISA Kit 96T/48T
33629人神经营养因子3(NT-3)ELISA试剂盒人神经营养因子3(NT-3)ELISA Kit96T/48T
33630人的神经生长因子(NGF)ELISA试剂盒 Human Nerve growth factor,NGF ELISA Kit 96T/48T
33631人巨噬细胞炎性蛋白3β(MIP-3β/ELC/CCL19)ELISA试剂盒Human Macrophage Inflammatory Protein-3β,MIP-3β ELISA Kit 96T/48T
33632人巨噬细胞炎性蛋白3α(MIP-3α/CCL20)ELISA试剂盒Human Macrophage Inflammatory Protein-3α,MIP-3α ELISA Kit 96T/48T
33633人巨噬细胞炎性蛋白1β(MIP-1β/CCL4)ELISA试剂盒 Human Macrophage Inflammatory Protein-1β,MIP-1β ELISA Kit96T/48T
33634人巨噬细胞炎性蛋白1α(MIP-1α/CCL3)ELISA试剂盒Human Macrophage Inflammatory Protein-1α,MIP-1α ELISA Kit 96T/48T
33635人单核细胞趋化蛋白3(MCP-3/CCL7)ELISA试剂盒Human monocyte chemotactic protein 3,MCP-3 ELISA Kit96T/48T
33636人单核细胞趋化蛋白2(MCP-2/CCL8)ELISA试剂盒Human monocyte chemotactic protein 2,MCP-2 ELISA Kit 96T/48T
33637人单核细胞趋化蛋白1(MCP-1/CCL2/MCAF)ELISA试剂盒Human monocyte chemotactic protein 1/monocyte chemotactic and activating factor,MCP-1/MCAF ELISA Kit96T/48T
33638人胰岛素样生长因子结合蛋白3(IGFBP-3)ELISA试剂盒Human insulin-like growth factors binding protein 3,IGFBP-3 ELISA Kit 96T/48T
33639人胰岛素样生长因子结合蛋白2(IGFBP2)ELISA试剂盒Human insulin-like growth factors binding protein 2,IGFBP-2 ELISA Kit 96T/48T
33640人胰岛素样生长因子结合蛋白1(IGFBP-1)ELISA试剂盒Human insulin-like growth factors binding protein 1,IGFBP-1 ELISA Kit 96T/48T
33641人粒细胞巨噬细胞集落刺激因子(GM-CSF)ELISA试剂盒Human Granulocyte-Macrophage Colony Stimulating Factor,GM-CSF ELISA Kit96T/48T
33642人胶质细胞系来源的神经营养因子(GDNF)ELISA试剂盒Human glial cell line-derived neurotrophic factor,GDNF ELISA Kit96T/48T
33643人粒细胞集落刺激因子(G-CSF)ELISA试剂盒Human Granulocyte Colony Stimulating Factor,G-CSF ELISA Kit 96T/48T
33644人碱性成纤维细胞生长因子6(bFGF-6)ELISA试剂盒Human basic fibroblast growth factor 6,bFGF-6 ELISA Kit 96T/48T
33645人碱性成纤维细胞生长因子4(bFGF-4)ELISA试剂盒Human basic fibroblast growth factor 4,bFGF-4 ELISA Kit 96T/48T
33646人酸性成纤维细胞生长因子1(aFGF-1)ELISA试剂盒Human acidic fibroblast growth factor 1,aFGF-1 ELISA Kit 96T/48T
33647人凋亡相关因子配体(FASL)ELISA试剂盒Human Factor-related Apoptosis ligand,FASL ELISA Kit 96T/48T
33648人凋亡相关因子(FAS/CD95)ELISA试剂盒Human Factor-related Apoptosis,FAS ELISA Kit 96T/48T
33649人嗜酸粒细胞趋化蛋白Eotaxin 1(Eotaxin 1/CCL11)ELISA试剂盒Human Eotaxin 1 ELISA Kit 96T/48T
33650人内分泌腺来源的血管内皮生长因子(EG-VEGF)ELISA试剂盒Human Endocrine gland vascular endothelial growth factor,EG-VEGF ELISA Kit 96T/48T
33651人睫状神经营养因子(CNTF)ELISA试剂盒Human Ciliary Neurotrophic Factor,CNTF ELISA Kit 96T/48T
33652人CXC趋化因子受体1(CXCR1)ELISA试剂盒Human CXC-chemokine receptor 1,CXCR1 ELISA Kit96T/48T
33653人XC趋化因子受体1(XCR1)ELISA试剂盒Human XC-chemokine receptor 1,XCR1 ELISA Kit96T/48T
33654人二级淋巴组织趋化因子(SLC/CCL21)ELISA试剂盒 Human secondary lymphoid-tissue chemokine,SLC ELISA Kit96T/48T
33655人E钙粘着蛋白/上皮性钙黏附蛋白(E-Cad)ELISA试剂盒Human E-Cadherin,E-Cad ELISA Kit 96T/48T
33656人脑源性神经营养因子(BDNF)ELISA试剂盒Human Brain derived neurotrophic facor,BDNF ELISA Kit96T/48T
33657人神经调节蛋白1(NRG-1)ELISA试剂盒human Neuregulin 1,NRG-1 ELISA Kit96T/48T
33658人多巴胺D2受体(D2R)ELISA试剂盒Human dopamine D2 receptor,D2R ELISA Kit96T/48T
33659人神经元凋亡抑制蛋白(NAIP)ELISA试剂盒 Human neuronal apoptosis inhibitory protein,NAIP ELISA Kit96T/48T
33660人食欲素/阿立新B(OX-B)ELISA试剂盒Human Orexin B,OX-B ELISA Kit96T/48T
33661人促睡眠肽(DSIP)ELISA试剂盒Human delta sleep-inducing peptide,DSIP ELISA Kit96T/48T
33662人精氨酸加压素(AVP)ELISA试剂盒 Human arginine vasopressin,AVP ELISA Kit96T/48T
33663人垂体腺苷酸环化酶激活肽(PACAP)ELISA试剂盒Human pituitary adenylate cyclase activating polypeptide,PACAP ELISA Kit96T/48T
 33664人微管相关蛋白2(MAP-2)ELISA试剂盒Human microtubule-associated protein 2,MAP-2 ELISA Kit96T/48T
33665人神经丝蛋白(NF)ELISA试剂盒Human neurofilament protein,NF ELISA Kit96T/48T
ELISA试剂盒操作步骤
方法一)用于检测未知抗原的双抗体夹心法:
1. 包被:用0.05M PH9.牰碳酸盐包被缓冲液将抗体稀释至蛋白质含量为1~10μg/ml。在每个聚苯乙烯板的反应孔中加0.1ml,4℃过夜。次日,弃去孔内溶液,用洗涤缓冲液洗3次,每次3分钟。(简称洗涤,下同)。
2. 加样:加一定稀释的待检样品0.1ml于上述已包被之反应孔中,置37℃孵育1小时。然后洗涤。(同时做空白孔,阴性对照孔及阳性对照孔)。
3. 加酶标抗体:于各反应孔中,加入新鲜稀释的酶标抗体(经滴定后的稀释度)0.1ml。37℃孵育0.5~1小时,洗涤。
4. 加底物液显色:于各反应孔中加入临时配制的TMB底物溶液0.1ml,37℃10~30分钟。
5. 终止反应:于各反应孔中加入2M硫酸0.05ml。
6. 结果判定:可于白色背景上,直接用肉眼观察结果:反应孔内颜色越深,阳性程度越强,阴性反应为无色或极浅,依据所呈颜色的深浅,以“+”、“-”号表示。也可测O·D值:在ELISA检测仪上,于450nm(若以ABTS显色,则410nm)处,以空白对照孔调零后测各孔O·D值,若大于规定的阴性对照OD值的2.1倍,即为阳性。
方法二)用于检测未知抗体的间接法:   
用包被缓冲液将已知抗原稀释至1~10μg/ml,每孔加0.1ml,4℃过夜。次日洗涤3次。加一定稀释的待检样品(未知抗体)0.1ml于上述已包被之反应孔中,置37℃孵育1小时,洗涤。(同时做空白、阴性及阳性孔对照)于反应孔中,加入新鲜稀释的酶标第二抗体(抗抗体)0.1ml,37℃孵育30-60分钟,洗涤,zui后一遍用DDW洗涤。其余步骤同“双抗体夹心法”的4、5、6。


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